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. Author manuscript; available in PMC: 2019 Jun 1.

Published in final edited form as: Radiat Res. 2018 Mar 27;189(6):560–578. doi: 10.1667/RR14878.1

FIG. 8 — Suppression of distant marrow hematopoietic colony-forming CFU-GM by head and neck irradiation of Fancg^−/− or Fanca^−/− mice. Bone marrow was removed from mice in each group (n = 5/group) in single-fraction (30 Gy) or fractionated irradiation (10 Gy × 4) neck irradiation protocols, at 5 days after the final radiation fraction. CFU-GM assay was performed, as described Materials and Methods. Panel A: Fancg^−/− and control mice in single-fraction irradiation protocol; panel B: Fancg^−/− and control mice in fractionated irradiation protocol; panel C: Fanca^−/− and control mice in single-fraction irradiation protocol; panel D: Fanca^−/− and control mice in fractionated irradiation protocol. The results are presented as a mean ± SEM of four CFU-GM cultures per mouse, 5 mice/group. P value compares each group to irradiation alone, as described in Materials and Methods. In panel A, ^πcompared to 0 Gy; *JP4-039 compared to 30 Gy; and ^#JP4-039 compared to 30 Gy + F15. In panel B, ^πcompared to 0 Gy; *JP4-039 compared to 10 Gy × 4; and ^JP4-039 compared to 10 Gy × 4 + 4-amino-Tempo/F15. In panel C, ^πcompared to 0 Gy; ^#JP4-039 compared to 30 Gy + F15; and ^JP4 compared to 30 Gy + 4-amino-Tempo/F15. In panel D, ^πcompared to 0 Gy, P < 0.05; *JP4-039 compared to 4 × 10 Gy.

FIG. 8 — Suppression of distant marrow hematopoietic colony-forming CFU-GM by head and neck irradiation of Fancg^−/− or Fanca^−/− mice. Bone marrow was removed from mice in each group (n = 5/group) in single-fraction (30 Gy) or fractionated irradiation (10 Gy × 4) neck irradiation protocols, at 5 days after the final radiation fraction. CFU-GM assay was performed, as described Materials and Methods. Panel A: Fancg^−/− and control mice in single-fraction irradiation protocol; panel B: Fancg^−/− and control mice in fractionated irradiation protocol; panel C: Fanca^−/− and control mice in single-fraction irradiation protocol; panel D: Fanca^−/− and control mice in fractionated irradiation protocol. The results are presented as a mean ± SEM of four CFU-GM cultures per mouse, 5 mice/group. P value compares each group to irradiation alone, as described in Materials and Methods. In panel A, ^πcompared to 0 Gy; *JP4-039 compared to 30 Gy; and ^#JP4-039 compared to 30 Gy + F15. In panel B, ^πcompared to 0 Gy; *JP4-039 compared to 10 Gy × 4; and ^JP4-039 compared to 10 Gy × 4 + 4-amino-Tempo/F15. In panel C, ^πcompared to 0 Gy; ^#JP4-039 compared to 30 Gy + F15; and ^JP4 compared to 30 Gy + 4-amino-Tempo/F15. In panel D, ^πcompared to 0 Gy, P < 0.05; *JP4-039 compared to 4 × 10 Gy.