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. Author manuscript; available in PMC: 2019 Jan 1.
Published in final edited form as: Neurobiol Dis. 2017 Oct 3;109(Pt A):127–136. doi: 10.1016/j.nbd.2017.10.002

FIGURE 4.

FIGURE 4

Ube2W deficiency increases soluble Htt levels in HdhQ200 KI mice

A. Western Blot of brain lysates from WT, Ube2W KO, HdhQ200 KI, or HdhQ200 KI/Ube2W KO mice. Immunoblot were performed with anti-polyQ (1C2), anti-Htt, anti-Ube2W and anti-GAPDH antibodies. (40 μg total protein/lane.)

B. Quantification of Blot against Htt in panel A.

C. Western Blot of soluble and insoluble fraction of mouse brain lysates from WT, Ube2W KO, HdhQ200 heterozygous (het) KI, HdhQ200 het KI with Ube2W KO mice. Blotted with anti-Htt antibody.

D. Quantification of HMW Htt blotted in panel C.

Arrowheads represent mutant HttQ200, arrow represents WT Htt, and bracket indicates high molecular weight (HMW) Htt in stacking gel. Graphs show means +/− SEM; *, p<0.05; **, p<0.01; ***, p<0.001. n=3.