Table 1. Specificity of the RT-LAMP assay.
| qRT-PCR | RT-LAMP | |||||
|---|---|---|---|---|---|---|
| Virus | Sample identification (copies/mL) | CP values | CP values | Genie II | ESE-Quant Tube Scanner | |
| min:s | Annealing (oC) | min | ||||
| CHIKV-PCR-EQA 07–2014 ENIVD | #10 CHIK Caribbean (2.2x104) | nda | nd | 58:00 | 83.26 | 59.7 |
| #12 CHIK Caribbean (1.3x105) | 34.7 | 32.59 | nd | nd | 26.7 | |
| #4 CHIK Caribbean (1.8x106) | 32.66 | 35.9 | 41:30 | 84.66 | 26.3 | |
| #9 CHIK Caribbean (2.4x107) | 28.78 | nd | 32:30 | 84.76 | 23.3 | |
| #2 CHIK Caribbean (1.8x108) | 25.94 | 26.6 | 28:45 | 84.74 | 20.7 | |
| #6 CHIK India (1.1x108) | 25.51 | 26.85 | 30:45 | 86.1 | 22.7 | |
| #7 CHIK Seychelles (2x108) | 25.37 | 26.88 | 31:15 | 85.95 | 23.3 | |
| #11 ONNV (1.8x104) | nd | nd | nd | nd | nd | |
| #8 DENV2 (8.3x104) | nd | nd | nd | nd | nd | |
| #1 SINDV (7.5x106) | nd | nd | nd | nd | nd | |
| #3 negative (plasma) | nd | nd | nd | nd | nd | |
| #5 negative (plasma) | nd | nd | nd | nd | nd | |
| DENV | ATCC VR-344, DENV1 | nd | nd | nd | nd | nd |
| ATCC VR-345, DENV2 | nd | nd | nd | nd | nd | |
| ATCC VR-1256, DENV3 | nd | nd | nd | nd | nd | |
| ATCC VR-1257, DENV4 | nd | nd | nd | nd | nd | |
| YFV | YFV | nd | nd | nd | nd | nd |
| WNV | WNV | nd | nd | nd | nd | nd |
| NTAV | NTAV | nd | nd | nd | nd | nd |
| SAV | Salmonid alphavirus, F02-143 | nd | nd | nd | nd | nd |
a non-detected