Skip to main content
. 2018 May 29;13(5):e0197751. doi: 10.1371/journal.pone.0197751

Fig 5. 2-AG promotes B6MCL macrophage migration as assessed by modified Boyden chamber experiments.

Fig 5

B6MCL macrophage migration was assessed by modified Boyden chamber experiments. 350,000 cells were seeded per well and allowed to migrate for 90 minutes. Cells adherent to the lower side of the polycarbonate membrane were counted automatically using Image J 1.48v software following DAPI staining. Addition of 2-AG [1 μM] to the lower chamber resulted in an increased migration of B6MCL macrophages (B) compared to DMSO (A). This effect was blunted by addition of CB1-inhibitor AM281 [1 μM] and by CB2-inhibitor AM630 [1 μM] (C). Data are presented as mean ± standard error of the mean; n = 4–6; *, p < 0.05, assessed by ANOVA and Bonferroni correction. Scale bar, 300 μm. 2-AG, 2-arachidonoylglycerol; AM281, selective inhibitor of CB1; AM630, selective inhibitor of CB2; DMSO, dimethyl sulfoxide.