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(A) RT-PCR using D1 and D2 primers. (B) Serotype-specific nested PCR using D1/TS1, D1/TS3 and D1/TS4 primers to detect DENV1, DENV3 and DENV4, respectively. L: 100 bp DNA ladder (Thermo Scientific); NC: negative control (H2O); 1: KDH0030A (DENV1); 2: DJOS1.7.12 (DENV2); 3: KDH0010A (DENV3); 4: VIMFH4 (DENV4); nP: negative control nested PCR; P: negative control PCR.
