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A phylogenetic tree between CPKs from maize and those functionally identified in pollen tube growth was established using the neighbor-joining method in MEGA ver. 4.0 software. The four subgroups are labeled as I, II, III, and IV, and ZmCPK32 is highlighted in red. The accession numbers used for the phylogenetic analysis are listed in the methods. The scale bar corresponds to a distance of 10 changes per 100 amino acid positions.
