(A) Expression of ZmCPK32 in different organs and developing seeds. The transcript levels of ZmCPK32 in vegetative tissues, including root, stem, leaf, and leaf sheath, reproductive tissues such as tassel, cob, silk, and anther, and the embryo (E) and endosperm (En) at the indicated days after pollination (13–23 DAP) were examined by qRT-PCR. (B) Expression profiles of ZmCPK32 in the developing tassel. The whole tassel development process was divided into eight stages, including the immature tassel stage, according to its length, and tassels were collected on the day of flowering (0 DAF) and branch expansion. ZmActin1 expression was used as an internal control, and the error bars indicate standard deviation.