Table 3. Procedure used for staining CD antigens in lymphocyte suspensions.
A | B | C | D | E | F | G |
---|---|---|---|---|---|---|
negative control | Background control | Background control | CD2/CD21 | CD4/CD8 | CD5/CD8 | TCRγδ/CD8 |
lymphocyte suspension | lymphocyte suspension | lymphocyte suspension | lymphocyte suspension | lymphocyte suspension | lymphocyte suspension | lymphocyte suspension |
1 μl S1 | 1 μl S2 | 1 μl P1 + 1 μl S2 | 1 μl P2 + 1 μl S3 + 1 ml S-PE | 1 μl P3 + 1 μl S1 | 1 μl P6 + 1 μl S1 | |
Washing | Washing | washing | Washing | washing | washing | washing |
FA | FA | FA | 1 μl P5 + 1 μl S1 | 1 μl P4 + 1 μl S3 | 1 μl P4 + 1 μl S3 | 1 μl P4 + 1 μl S3 |
END | END | END | Washing | washing | washing | washing |
FA | FA | FA | FA | |||
END | END | END | END |