Skip to main content
. 2018 May 29;13(5):e0196458. doi: 10.1371/journal.pone.0196458

Table 3. Procedure used for staining CD antigens in lymphocyte suspensions.

A B C D E F G
negative control Background control Background control CD2/CD21 CD4/CD8 CD5/CD8 TCRγδ/CD8
lymphocyte suspension lymphocyte suspension lymphocyte suspension lymphocyte suspension lymphocyte suspension lymphocyte suspension lymphocyte suspension
1 μl S1 1 μl S2 1 μl P1 + 1 μl S2 1 μl P2 + 1 μl S3 + 1 ml S-PE 1 μl P3 + 1 μl S1 1 μl P6 + 1 μl S1
Washing Washing washing Washing washing washing washing
FA FA FA 1 μl P5 + 1 μl S1 1 μl P4 + 1 μl S3 1 μl P4 + 1 μl S3 1 μl P4 + 1 μl S3
END END END Washing washing washing washing
FA FA FA FA
END END END END