Fig 1. In vitro characterization of PDGFRβ-selected cells isolated from lungs of Pdgfrb-Cre+;Itga8^flox/- (Cre+) and WT or Itga8^flox/flox (Cre-) mice.

(A) Western blot showing the absence of ITGA8 expression in Cre+ cells compared to Cre- cells. (B) Adhesion assay showing decreased binding to the substrates nephronectin (NPNT) and fibronectin (FN) by Cre+ cells. (C-G) PDGFRβ-selected cells were treated with TGFβ (10 ng/ml) or vehicle. mRNA expression is presented as fold change relative to Cre- in the untreated group (vehicle). Genes include: Itga8 (C), Col1a1 (D), Acta2 (E), Ctgf (F), and Fn1 (G). (*p<0.05, mean±SEM, n = 4).