Fig 3. raw is required to prevent cell death along peripheral nerves.

(A-D) Maximum intensity projections of peripheral nerves of third instar larvae co-stained for cells undergoing apoptosis (anti-cleaved Death caspase-1, red) and glia (anti-Repo, green). Posterior to the right. (A) Control animals (repo-Gal4; n = 8). (B) Control animals overexpressing dcr2 (repo-Gal4>dcr2; n = 9). (C) Panglial raw knockdown does not increase cell death (repo-Gal4>raw-RNAi²; n = 10). (D) Panglial raw knockdown in the presence of overexpressed dcr2 results in cell death along peripheral nerves (repo-Gal4>raw-RNAi², dcr2; n = 9). Arrows indicate dying cells. Scale bars are 50μm.