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. 2018 May 16;14(5):e1007382. doi: 10.1371/journal.pgen.1007382

Fig 5. Condensin I affects both meiotic cohesins.

Fig 5

(A) Immunofluorescence images of REC-8 or COH-3/4, as well as SC components SYP-1 and HTP-3 in rec-8 or coh-4 coh-3 mutants treated with control vector or capg-1 RNAi in early pachytene (EP) or late pachytene (LP). Levels of both cohesins are reduced, and SYP-1 levels are reduced in coh-4 coh-3 mutants after condensin I depletion. Scale bar, 5 μm. (B) Quantification of REC-8 signal on chromosomes of coh-4 coh-3 mutants. The proportion of DNA (DAPI) signal overlapping with REC-8 signal is significantly decreased in capg-1(RNAi) (p<0.001, Student’s t-test). (C) 5S rDNA FISH analysis of early and late pachytene nuclei in rec-8 or coh-4 coh-3 mutants with control vector or capg-1(RNAi). (D) Graphs showing the percentage of nuclei with 1, 2, 3, or 4, 5S rDNA signals. Asterisks indicate statistical significance using Chi square test with three categories (1 focus, 2 foci, 3 or 4 foci). ** indicates p<0.01, *** indicates p<0.001. Numbers of nuclei analyzed and p values are shown in S1 Table.