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CID MS3 spectra of the disulfide-dissociated peptides TL2 (top) and TL6 peptides (bottom) within the VL domain. The C89 residue in TL6 was identified by MS3 spectrum with high confidence. The identity of TL2 was confirmed by the mass difference between the disulfide-linked peptide and the disulfide-dissociated peptide. The partial MS3 sequence coverage further confirmed the linkage between C22 (L) and C89 (L).
