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CID MS3 spectra of the disulfide-dissociated peptides TL17 (top) and TH15 peptides (bottom) across the CL and CH1 domain. TL17 is at the C-terminal of the light chain of the mAb, ending with S. Three or one fragment ion was missing for CID MS3 spectrum of TH15 or TL17 to achieve an MS3 sequence coverage of 100%. Both C144 (H) and C215 (L) residues were identified by high confidence, and thus the location of disulfide linkages were unambiguously identified.
