Table 1.
Disulfide isoform distribution of the IgG2 mAb preparations
| Enriched for IgG2-A |
Enriched for IgG2-B |
||||
|---|---|---|---|---|---|
| Disulfide Isoforma | Starting Materialb | Redox onlyc | Redox + CEXd | Redox onlyc | Redox + CEXd |
| A + A’ | 22% | 65% | 100% | 16% | 0% |
| A/B | 33% | 26% | 0% | 14% | 0% |
| B | 45% | 9% | 0% | 70% | 100% |
a
Distribution of disulfide isoforms were determined via nrRP-UHPLC.
b
Initial IgG2 mAb sample (“starting material”) is comprised of a mixture of A, A/B, and B disulfide isoforms.
c
Distribution of disulfide isoforms after dialysis in redox buffer for 5 days at 4°C, protected from light.
d
Distribution of disulfide isoforms of redox-enriched A and B mAb further purified via CEX-HPLC fractionation.