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. Author manuscript; available in PMC: 2019 Mar 22.
Published in final edited form as: Cell. 2018 Mar 1;173(1):104–116.e12. doi: 10.1016/j.cell.2018.02.014

Figure 2. CDK1-CDK4-CCNB-CCND Induces EDU- and PHH3-Positive Human, Mouse and Rat Cardiomyocytes In Vitro.

Figure 2

(A) Representative immunocytochemistry images of PHH3, EDU, cardiac TroponinT (cTnT) and Dapi in 60-day-old human iPS-derived cardiomyocytes (iPS-CMs) 48 hours after control or CDK1-CDK4-CCNB-CCND (4F) viral infection, shown with higher magnification insets.

(B) Quantification of EDU+:cTnT+ or PHH3+:cTnT+ nuclei among CMs in response to overexpression of the indicated proteins in 60-day-old human iPS-CMs (n=3 independent experiments, *p<0.05).

(C) Representative immunocytochemistry of P7 primary mouse cardiomyocytes (top rows) and 4-month-old adult rat cardiomyocytes (lower rows) infected with LacZ (Control, top panel) or 4F (CDK1-CDK4-CCNB-CCND, lower panel) expressing adenoviruses and stained 48 hours later with PHH3 (green), cTnT (red) and DAPI nuclear stain (blue); insets represent higher magnification.

(D) Quantification of mouse or rat cTnT+ cardiomyocytes that were PHH3+ as a percentage of total cTnT+ cells (n=260 cells cTnT+ counted for each type). Bars represent average of three experiments and error bars indicate SEM (*p<0.05). Also, see Figure S1.