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. Author manuscript; available in PMC: 2019 Mar 22.
Published in final edited form as: Cell. 2018 Mar 1;173(1):104–116.e12. doi: 10.1016/j.cell.2018.02.014

Figure 4. Cardiomyocytes Degrade Overexpressed Cell-Cycle Proteins over Time in a Proteasome-Dependent Manner.

Figure 4

(A) Quantification of single-colored cells in α-MHC-Cre MADM mice 1, 2 and 3 weeks after coronary ligation and 4F infection. After the initial increase in number of single-colored cells during the first week, there was no further increase (n=600 cells analyzed from four animals in each group).

(B) Bar graphs show RNA expression (left panel) and protein expression (right panel) of the overexpressed cell cycle genes at 2 and 6 days after infection (n=3, *p<0.05).

(C) Representative immunofluorescence images and quantification for indicated protein (green) and cardiac Troponin-T (red) over time demonstrates a decline in the overexpressed protein expression by day 6 after adenoviral infection of each (n=3 independent experiments, *p<0.05).

(D) Representative images (for CCNB) and quantification of cTnT+ cardiomyocytes treated with proteosome inhibitors (Bortezomib or MG132) for 48 hours starting at day 4 demonstrate persistent protein expression of the overexpressed cell cycle genes at day 6 (n=3, *p<0.05) in the presence of proteosome inhibitors. Bars indicate means and SEM. Also, see Figure S7.