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. Author manuscript; available in PMC: 2018 May 29.
Published in final edited form as: Mol Neurobiol. 2013 Oct 4;49(2):796–807. doi: 10.1007/s12035-013-8562-z

Fig. 4.

Fig. 4

Co-localization of overexpressed wild-type SOD1, SOD1 (G93A) mutant, and PDI in NSC-34 cells. Cells receiving the PDI construct were stained immunocytochemically 48 h after transfection. Images of wild-type SOD1 (green), SOD1 (G93A) mutant (green), and PDI (red) and merge were monitored under confocal microscopy. Wild-type SOD1 stained diffusely inside of cells, consistent with its previously reported cytosolic distribution (a), while mSOD1 did not stain the nucleus, indicating its nuclear-sparing pattern of distribution (b). Wild-type and m SOD1 coprecipitate with PDI and induce PDI expression in NSC-34 cells. The anti-SOD1 antibody coprecipitated PDI, and the anti-PDI antibody coprecipitated SOD1 in NSC-34 cells transfected with wild-type SOD1 or m SOD1. SOD1 thus interacts with PDI in NSC-34 cells transfected with wild-type SOD1 or m SOD1 (c)