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. Author manuscript; available in PMC: 2018 May 29.
Published in final edited form as: Cell Chem Biol. 2018 Feb 22;25(4):460–470.e6. doi: 10.1016/j.chembiol.2018.01.013

Figure 3. The Mass Spectrum Labeling of SRPKIN-1 with SRPK1 Protein.

Figure 3

(A) Electropherograms (left), mass spectra (middle), and zero-charge mass spectra (right, overlayed) of SRPK1 protein treated with DMSO (top, red) or 10-fold molar excess of SRPKIN-1 (bottom, green) for 2 hr at room temperature. The observed mass shift of 452 Da is consistent with covalent labeling by SRPKIN-1 (with elimination of HF).

(B) Tandem MS spectrum of tryptic SRPK1 peptide (residues 209–229) with Y227 modified by SRPKIN-1. Ions of type b and y are indicated with blue and red glyphs, respectively. Y*, SRPKIN-1-modified tyrosine.