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. Author manuscript; available in PMC: 2018 May 29.
Published in final edited form as: Cell. 2018 Feb 22;172(5):966–978.e12. doi: 10.1016/j.cell.2018.02.009

Figure 3. CryoEM helical reconstruction of eNP nucleocapsid-like structure.

Figure 3

(A) Raw cryoEM micrograph. Scale bar represents 500 Å. (B) An asymmetric unit of the tubular structures with two eNP-2 molecules (mol. a, blue; mol. b, cyan) relative to each other without any n-fold symmetry operation. The translation and rotation parameters to superimpose eNP-2 molecules are shown. The extended helix (α23) of eNP-2 molecule b occupies the eVP35 NPBP binding pocket of eNP-2 molecule a. (C) 3D reconstruction of masked eNP structure shows a left-handed hollow tube. Left. Side view. Three asymmetric units colored yellow/brown, cyan/blue, and pink/magenta containing six eNPs are highlighted. Right. Top view. Outer and inner diameters are 490 Å and 330 Å, respectively. (D) Views of three eNPs in the highlighted bottom row in (C), rotated 125° along the same direction of the same rotation axis illustrated in (C). These eNPs lack the density for helix α23 observed in eNPs in (E). (E) Views of three eNPs in the highlighted top row in (C), rotated 70°along the same direction of the same rotation axis illustrated in (C). The coiled-coil motif-like contacts between helices α21 and α22 and position of helix α23 are shown. See also Figure S3–S4 and Movies S1–3.