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Electron cryomicroscopy data collection and processing of eNP-2 structure
| Microscope | JEM3200FSC |
| Voltage (kV) | 300 |
| Energy filter slit width (eV) | 30 |
| Detector | Gatan K2 Summit |
| Magnification | 30,000X |
| Pixel size (Å) | 1.2 |
| Defocus range (μm) | 0.5 – 2.0 |
| Electron exposure (e−/Å2) | 24 |
| Micrographs (acquired/used) | 1266 / 1113 |
| Segment step (asymmetric unit) | 5 |
| Number of segments/asymmetric unit | 169,526 / 847,630 |
| Helical twist and rise (°/Å) | −8.53 / 2.65 |
| Map resolution at 0.143 FSC criterion (Å) | 5.8 |
