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. 2017 Jul 8;9(37):24653–24671. doi: 10.18632/oncotarget.19065

Figure 6. Collagen increases Kv10.1 functional channel activity in BC cells.

Figure 6

(A) Whole cell currents recorded in cells treated or not with collagen 1. Cells were starved for 48 h and the patch-clamp measurements were performed. 500 msec voltage ramps from -100 to +80 mV from a holding potential of -40 mV were applied to record Kv10.1 channel activity in MCF-7 (a) and T-47D (b) cell lines. The collagen-activated outward current is shown in the small squares. (B) Astemizole-sensitive current traces in MCF-7 (a) and T-47D (b) cells seeded or not on collagen 1 obtained from the subtraction of the current remained after astemizole perfusion from the whole cell current. (C) siKv10.1 sensitive current in MCF-7 (a) and T-47D (b) cells treated or not with collagen, obtained from the subtraction of the whole cell current recorded in cells transfected with siKv10.1 from the average whole cell current recorded in cells transfected with si-control. Values are reported as mean. *p <0.05, **p< 0.01. Mann-withney test. (D) Effect of the perfusion of astemizole (5 μM) on the outward current recorded in siKv10.1-tranfected MCF-7 (a) and T-47D (b) cells seeded on collagen 1. Astemizole failed to affect the amplitude of the remaining outward current in siKv10.1-transfected cells.