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. 2018 May 15;9(37):24718–24730. doi: 10.18632/oncotarget.25339

Figure 1. Effects of resveratrol on miRNA and mRNA profiles and resveratrol-regulated miRNAs in breast cancer.

Figure 1

(A) Heat map showing miRNAs whose expression levels differed by >1.2-fold in MDA-MB-231-luc-D3H2LN cells, with or without resveratrol treatment (P < 0.1). Twenty-four miRNAs were up-regulated and 22 miRNAs were down-regulated by resveratrol treatment. (B) The eight most highly up-regulated miRNAs following resveratrol treatment, as determined by microarray analysis. miRNA expression was validated by qRT-PCR in MDA-MB-231-luc-D3H2LN and MCF7 cells (mean ± SD, *P < 0.05, **P < 0.01). n.d.: not determined (C) Heat map showing mRNAs that differed by >1.5-fold in MDA-MB-231-luc-D3H2LN cells, with or without resveratrol treatment (P < 0.01). A total of 2,046 mRNAs were up-regulated and 1,176 mRNAs were down-regulated by resveratrol treatment. (D) Gene set-enrichment analysis of the p53 pathway signature in MDA-MB-231-luc-D3H2LN cells treated with resveratrol, as compared with control cells (treated with vehicle). NES: normalized enrichment score (E) GFP signals in transgenic MCF7 cells expressing control non-target shRNA and TP53-knockdown shRNA. MCF7/shNC: MCF7/H1::non-target shRNA/CMV::copGFP and MCF7/shp53: MCF7/H1::TP53 shRNA/CMV::copGFP. (F) Immunoblot analysis of p53 expression in MCF7 cells expressing a control non-target shRNA and p53-knockdown shRNA. (G) The expression of resveratrol-regulated miRNAs in p53-knockdown MCF7 cells and control cells, with or without resveratrol treatment (mean ± SEM, *P < 0.05). Two independent clones were prepared for each cell line. The number of data points shown for each sample is four.