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. 2018 May 29;9(6):651. doi: 10.1038/s41419-018-0699-2

Fig. 5. Function of circ-HIPK2 during ATRA-induced differentiation of NB4 cells.

Fig. 5

a Schematic illustration of circ-HIPK2 from the HIPK2 gene region and siRNA designed according to the junction sequence of circ-HIPK2. b qRT-PCR was performed to detect the expression of circ-HIPK2 and linear-HIPK2 in NB4 cells transfected with circ-HIPK2 siRNA or negative control siRNA. c Proliferation detection of NB4 cells after transfected with si-circ-HIPK2 at the indicated time. d Downregulation of circ-HIPK2 reduced the ATRA-induced differentiation of NB4 cells. e The expression of circ-HIPK2 and linear-HIPK2 in NB4 cells transfected with pcDNA-GAPDH or pcDNA-CHIPK2. f Overexpression of circ-HIPK2 increased the differentiation ratio of NB4 cells, with less effect in cells treated with ATRA simultaneously. The data are shown with the means ± s. e. m. of three independent biological experiments. ***P < 0.001