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. 2018 May 29;8:8244. doi: 10.1038/s41598-018-26472-8

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© The Author(s) 2018

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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PDCM-CB-P grafts enabled stable peri-implant bone regeneration in rabbit tibias. (A–F) Merged images of all test groups showed obvious differences in bone formation. Bar = 1 mm, magnification = 4×. (G,M) Bone regeneration around the implant, as examined by histological analyses, including (H,E), and Masson’s trichrome staining. Bar = 200 μm, magnification = 10×. (H–L, N–R) Similar staining showing much less cancellous bone formation and clear PDCM or fibrous tissues remaining. Bar = 200 μm, magnification = 10×. PDCM, particulate decellularised cartilage matrix; CB, cell brick; P, platelet-rich plasm.