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. 2018 May 29;8:8316. doi: 10.1038/s41598-018-26704-x

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© The Author(s) 2018

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Effects of HGF on migration and proliferation of Schwann cells. Primary Schwann cells isolated from rat sciatic nerves were treated with recombinant human HGF protein to measure the effects on migration and proliferation. (A) Expression of c-met receptor in primary SCs. Scale bar = 25 μm. Full-length blots are presented in Supplementary Fig. S6. The samples derived from the same experiment and blots were processed in parallel. (B) Effects of HGF on SC migration. Primary SCs were treated with 10 ng/ml and 25 ng/ml of recombinant HGF protein, and their migration was analyzed using Boyden chambers. ****p < 0.0001. Values represent the mean ± S.E.M. of three independent experiments. (C) Effect of HGF on proliferation of SCs. SCs were treated with 5, 10, 25, 50, and 100 ng/ml HGF and SC proliferations were analyzed by WST-1 assay. (D) SCs proliferation was analyzed by immunocytochemistry assay, using an antibody to Ki67 (Red). Scale bar = 50 μm. (E) Signaling cascades activated by HGF treatment in SCs. Primary Schwann cells isolated from rat sciatic nerves were treated with recombinant human HGF protein. Total proteins were isolated from SCs treated with HGF at appropriate times and analyzed by Western blot using antibodies to respective proteins. Full-length blots are presented in Supplementary Fig. S7. The samples derived from the same experiment and blots were processed in parallel.