Fig. 3. Protein and mRNA expression of Dnmt1 in PDAC mouse models.

a RT-qPCR of Dnmt1 mRNA abundance in primary mouse tissues. The ΔΔCT method was used to calculate fold change using β-actin as the reference gene with the fold change being relative to the Kras Pten^flox samples. The * represent non-specific bands of unknown origin. b RT-qPCR of Dnmt1 expression in primary cell lines. The ΔΔCT method was used to calculate fold change with β-actin as the reference gene with fold change normalised to the Kras Pten^flox cell line. The result represents the mean of three biological repeats with the error bars representing the maximum relative quantity. A two-sample, two-tailed, paired t-test was used to compare the ΔCT values from each group. Statistical significance is represented as *p < 0.05 and ***p < 0.005. c A representative western blot showing the expression of Dnmt1, Pten, p53 and β-actin in the primary cell lines