Fig. 4. Expression of microRNAs following treatment with 5-aza-deoxycytidine.
The Kras p53R172H/+ cell line was treated with 1 µm 5-aza-deoxycytidine (5-aza-Dc) or an equal volume of a vehicle control (DMSO) for 24 h. The expression of the microRNAs identified as dysregulated in p53R172H-expressing tissues was quantified by RT-qPCR with U6 snRNA as the reference gene. The result represents the average of three biological repeats with the error bars representing the maximum relative quantity. A two-sample, two-tailed, paired t-test was used to compare the ΔCT values from each group. Statistical significance is represented as *p < 0.05 and ***p < 0.005