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. 2018 Jan 18;11(3):210–220. doi: 10.1007/s12265-018-9789-x

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© The Author(s) 2018

Open Access This article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made.

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Fig. 2 — Gelatin zymography results. a Scatter plot of serum MMP-9 levels measured by ELISA and total MMP-9 intensities analyzed by gelatin zymography. The correlation coefficient and p value are shown. b Representative gelatin-zymography of ACS serum samples. Lane 1 is the molecular weight standard. Lanes 2, 4, and 6 are serum samples of patients with ACS without MMP-9 activating pretreatment. Lanes 3, 5, and 7 are the same serum samples, respectively, with 1 mM APMA pretreatment, which activates the pro-form of MMP-9. The gels are 10%, and the bands were visualized by Coomassie Brilliant Blue staining. Pro-MMP-2 bands are seen at 72 kDa. No proteolytically activated MMP-2 was observed (64 kDa)