Table 3.
Statistical results of the laboratory experiment. Parameters tested include maximum photochemical efficiency (Fv/Fm), and effective photochemical efficiency (∆F/Fm’), photosynthetic rate (P), respiration rate (R), the ratio P:R, Symbiodinium density (Symb.), chlorophyll a and c density (chl), protein content, and ash-free dry weight (AFDW).
| Trans. | Out. rem. | Treatment (num. df = 1) | Temperature (num. df = 7) | Treatment: Temperature (num. df = 7) | |||||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
| SS | MS | D. df | F | p | SS | MS | D. df | F | p | SS | MS | D. df | F | p | |||
| Photosynthesis | |||||||||||||||||
| Fv/Fm | p/(1 − p) | N | 18.9 | 18.9 | 686.1 | 676.4 | <0.01 | 35.5 | 5.1 | 679.0 | 181.9 | <0.01 | 25.1 | 3.6 | 677.9 | 128.5 | <0.01 |
| ∆F/Fm’ | p/(1 − p) | N | 0.7 | 0.7 | 9.8 | 106.2 | <0.01 | 23.1 | 3.3 | 674.0 | 106.2 | <0.01 | 11.3 | 1.6 | 672.5 | 52.0 | <0.01 |
| Gross P | — | N | <0.1 | <0.1 | 121.4 | 4.0 | <0.01 | 0.3 | <0.1 | 24.1 | 128.3 | <0.01 | <0.1 | <0.1 | 7.6 | 124.7 | <0.01 |
| R | — | Y | <0.1 | <0.1 | 121.0 | 18.9 | <0.01 | <0.1 | <0.1 | 128.8 | 14.9 | <0.01 | <0.1 | <0.1 | 125.0 | 3.0 | 0.01 |
| Gross P:R | — | Y | 14.3 | 14.3 | 119.9 | 41.3 | <0.01 | 25.3 | 3.6 | 10.5 | 126.2 | <0.01 | 12.9 | 1.8 | 5.3 | 122.8 | <0.01 |
| Sponge condition | |||||||||||||||||
| Symb. | — | N | 18944 | 18944 | 125.2 | 8.5 | <0.01 | 173824 | 24823 | 129.7 | 11.1 | <0.01 | 89443 | 12778 | 127.4 | 5.7 | <0.01 |
| Chl a | — | N | 25624 | 25624 | 123.2 | 19.6 | <0.01 | 121098 | 17300 | 13.2 | 128.4 | <0.01 | 94888 | 13556 | 10.4 | 13556 | <0.01 |
| Chl c | — | N | 990 | 990 | 123.1 | 20.3 | <0.01 | 7072 | 1010 | 20.8 | 128.1 | <0.01 | 4372 | 624.6 | 12.8 | 125.2 | <0.01 |
| Chl a:c | Log | Y | <0.1 | <0.1 | 123.3 | 0.4 | 0.52 | 0.8 | 0.1 | 5.3 | 128.6 | <0.01 | 0.1 | <0.1 | 0.8 | 126.1 | 0.59 |
| Protein | Log | N | 0.8 | 0.8 | 120.5 | 10.4 | <0.01 | 4.0 | 0.6 | 7.9 | 130.2 | <0.01 | 2.3 | 0.3 | 4.6 | 124.8 | <0.01 |
| AFDW | Log | Y | 0.3 | 0.3 | 122.1 | 14.3 | <0.01 | 0.7 | 0.1 | 5.8 | 129.2 | <0.01 | 0.7 | 0.1 | 5.5 | 125.0 | <0.01 |
Units for each measure are provided in Figs 3–4. Data were analysed using a linear mixed model with four components: temperature treatment, temperature, treatment:temperature interaction, and a random intercept for the C. orientalis sponge. Symbiodinium density was analysed with an additional random intercept for each aquarium. Parameters that were significantly affected by the temperature exposure have a significant treatment:temperature interaction term. The table includes the transformation used (Trans.), whether any outlying observations were removed, and statistical estimates: sums of squares (SS), mean square (MS), denominator degrees of freedom (D. df), F ratio (F), and P value (p).