Fig. 3.

Segment contain the minor alleles of the tagged SNPs increased the transcriptional activity using luciferase reporter assay. The segments of the putative regulatory region containing the three candidate loci were inserted into the PGL-3 basic vector. Then the two vectors were transfected into the 293T cell line. The pRL-TK vector encoding the Renilla luciferase was transcribed, which was used as internal controls to normalize firefly luciferase expression. The error bars denote the SD of the relative activities. Comparisons were performed, and the P-values are labeled in the plot