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. 2018 May 29;8:8285. doi: 10.1038/s41598-018-26699-5

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© The Author(s) 2018

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Quantification of the thickness of cell clumps formed by Acinetobacter sp. Tol 5 on a rectangular observation cell at different shear stresses. (A) Confocal laser scanning microscopy 3D-images with the x-z and y-z projections after flowing 7 mL of cell suspensions of Tol 5 WT and ΔataA mutant cells (OD₆₆₀ = 0.2). The red arrows indicate flow direction. Scale bar 20 µm. (B) Quantification data of the thickness of cell clumps formed by Tol 5 WT (black) and ΔataA mutant (white) cells. Data are expressed as the mean ± standard error from three independent flows (n = 3). *P < 0.01.