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. 2018 May 23;11:168. doi: 10.3389/fnmol.2018.00168

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Copyright © 2018 Antonelli, Casciati, Tanori, Tanno, Linares-Vidal, Serra, Bellés, Pannicelli, Saran and Pazzaglia.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Shh pathway deregulation alters the cell stage composition of DG. (A) Schematic representation of adult neurogenesis in the hippocampal DG and relative stage specific markers. (B,C) Representative immunostaining images and quantification for glial fibrillary acidic protein (GFAP). (D,E) Sex determining region Y (SRY) box 2 (Sox2), (F,G) Ki67 and (H,I) doublecortin (DCX), (J,K) NeuN. Images, 10× magnification, scale bar = 100 μm; 20× magnification, scale bar = 50 μm; 100× magnification, scale bar = 10 μm. The number of mice used per test is indicated in the graphs (n). Data are reported as mean ± SEM *p < 0.05; **p < 0.01 for comparison with controls (Student-t test).