Figure 6.

Complement activation induced by the domain muteins. (A) C1q binding to the domain muteins (O10 variants). The data used for calculations are presented in Figure S7 in Supplementary Material. Error bars correspond to uncertainty calculated as described in Section “Materials and Methods.” (B) Complement cascade activation by the domain muteins. Erythrocytes coated with 3 µg/ml of the muteins were incubated with complement serum. 100% lysis corresponds to water-induced lysis. The bars present mean values and standard deviation of duplicates from one experiment. Results obtained with 1.5 µg/ml of the muteins are presented in Figure S8 in Supplementary Material. (A,B) Representative results of two independent experiments.