Table 2.
Scores of hemagglutination induced by M18 variants.
| Conc. (μg/ml) | Parental IgGs | Swap of hinge regions | Swap of CH1 + hinge domains | Swap of CH2 domains | Swap of CH3 domains | |||||||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
| IgG1 | IgG3 | IgG1_h-3 | IgG3_h-1 | IgG1_CH1h-3 | IgG3_CH1h-1 | IgG1_CH2-3 | IgG3_CH2-1 | IgG1_CH3-3 | IgG3_CH3-1 | |||||||||||
| Ia | II | I | II | I | II | I | II | I | II | I | II | I | II | I | II | I | II | I | II | |
| 1.500 | – | – | +++ | ++ | – | – | +++ | +++ | – | – | +++ | +++ | + | + | – | + | – | ± | ++ | +++ |
| 0.750 | – | – | +++ | +++ | – | – | + | +++ | – | – | ++ | +++ | – | ± | – | – | – | – | + | ++ |
| 0.375 | – | – | ++ | +++ | – | – | ± | + | – | – | + | ++ | – | – | – | – | – | – | ± | ++ |
| 0.188 | – | – | + | ++ | – | – | – | – | – | – | ± | + | – | – | – | – | – | – | – | – |
| 0.094 | – | – | ± | ± | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
| 0.047 | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
aResults of two independent experiments designated as I and II.