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. 2018 May 29;8:8271. doi: 10.1038/s41598-018-26565-4

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© The Author(s) 2018

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Monitoring the time-varying distribution of intracellular paramylon in E. gracilis cells under glucose-sufficient and -deficient conditions. (A) Fluorescence images (top) and violin plots of the fluorescence intensity (bottom) of E. gracilis cells on Day 1, Day 2, Day 3, and Day 6. (B) Paramylon amount per cell on Day 1 and Day 6 measured by carbohydrate assay. (C) Relation between the paramylon amount per cell and the mean fluorescence intensity of E. gracilis cells. The mean fluorescence intensity at a paramylon amount of 0 ng indicates the background fluorescence intensity of the AF-6 medium without cells.