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. 2018 May 29;8:8224. doi: 10.1038/s41598-018-26667-z

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© The Author(s) 2018

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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PLOP-FISH signals in gymnosperm and angiosperm species. (a) Ginkgo biloba and (b) Pinus densiflora, (c) Hordeum vulgare, (d) Allium cepa, (e) Phaseolus vulgaris, (f) Trigonella foenum-graecum. Panels i–iv show the merged, raw 45S rDNA, raw 5S rDNA, and raw telomeric PLOP signals, respectively. Red, green, and blue signals in a-i – f-i represent 45S rDNA, 5S rDNA, and telomere, respectively. Red, green, and yellow arrows indicate weak 45S, weak 5S, and 45S signal crosstalk in the FITC filter, respectively. Bars = 10 μm.