Table 1.
List of oligoprobes used in this study.
| No. | Probe | Sequence | Length | Position in consensus | Tm (°C)* | Modification |
|---|---|---|---|---|---|---|
| 1 | 18SrDNA_UniOP_1 | CCGGAGAGGGAGCCTGAGAAACGGCTAC | 28 | 386…413 | 49.2 | 5′-Cy3 |
| 2 | 18SrDNA_UniOP_2 | ATCCAAGGAAGGCAGCAGGCGCGCAA | 26 | 417…442 | 50.9 | 5′-Cy3 |
| 3 | 18SrDNA_UniOP_3 | GGGCAAGTCTGGTGCCAGCAGCCGCGGT | 28 | 555…582 | 53.4 | 5′-Cy3 |
| 4 | 18SrDNA_UniOP_4 | TCGAAGACGATYAGATACCGTCSTAGT | 27 | 994…1020 | 40.4–43.4 | 5′-Cy3 |
| 5 | 18SrDNA_UniOP_5 | CTGAAACTTAAAGGAATTGACGGAAGG | 27 | 1133…1159 | 40 | 5′-Cy3 |
| 6 | 18SrDNA_UniOP_6 | GGAGCCTGCGGCTTAATTTGACTCAAC | 27 | 1174…1200 | 45.0 | 5′-Cy3 |
| 7 | 18SrDNA_UniOP_7 | GGTGGTGCATGGCCGTTCTTAGTTGGTGG | 29 | 1272…1300 | 47.1 | 5′-Cy3 |
| 8 | 18SrDNA_UniOP_8 | ACGTCCCTGCCCTTTGTACACACCGCCCGTC | 31 | 1622…1652 | 51.0 | 5′-Cy3 |
| 9 | 5.8SrDNA_UniOP_1 | AAYGACTCTCGGCAACGGATATCTMG | 26 | 16…41 | 42.0–44.9 | 5′-Cy3 |
| 10 | 5.8SrDNA_UniOP_2 | CWYGCATCGATGAAGAACGTAGCRA | 25 | 45…69 | 42.2–45.2 | 5′-Cy3 |
| 11 | 5.8SrDNA_UniOP_3 | GCGATACTTGGTGTGAATTGCAGAATC | 27 | 73…99 | 42.0 | 5′-Cy3 |
| 12 | 5.8SrDNA_UniOP_4 | GTGAACCATCGAGTYTTTGAACGCAAGT | 28 | 102…129 | 43.4 | 5′-Cy3 |
| 13 | 5SrDNA_ang_1 | GGATGCGATCATACCAGCACTAAAGCACCG | 30 | 1…30 | 48.2 | 5′-Alexa Fluor 488 |
| 14 | 5SrDNA_gym_1 | GRGTGCGATMATACCASCGYTWRYGYA | 27 | 1…27 | 38.4–48.0 | 5′-Alexa Fluor 488 |
| 15 | 5SrDNA_cranial_1 | GYYTAYRGCCAYACCACCCTGRRHRCG | 27 | 1…27 | 37.8–48.3 | 5′-Alexa Fluor 488 |
| 16 | 5SrDNA_ang_2 | CCCATCAGAACTCCGAAGTTAAGCGTGCT | 29 | 34…62 | 47.4 | 5′-Alexa Fluor 488 |
| 17 | 5SrDNA_gym_2 | ATCCSATCAGAACTCCGYARTTAAGCR | 27 | 32…58 | 39.7–45.6 | 5′-Alexa Fluor 488 |
| 18 | 5SrDNA_cranial_2 | GATCTCGTCYGATCTCGGAAGCTAAGC | 27 | 31…57 | 45.6–46.8 | 5′-Alexa Fluor 488 |
| 19 | 5SrDNA_ang_3 | GCGAGAGTAGTACTAGGATGGGTG | 24 | 66…89 | 40.7 | 5′-Alexa Fluor 488 |
| 20 | 5SrDNA_gym_3 | TTGGGYYRGAGTAGTACTRGGATGGGT | 27 | 62…88 | 38.4–42.2 | 5′-Alexa Fluor 488 |
| 21 | 5SrDNA_cranial_3 | GTCGGGCCYGGTYAGTACTTGGATGGG | 27 | 61…87 | 44.4–46.6 | 5′-Alexa Fluor 488 |
| 22 | 5SrDNA_ang_4 | CCTGGGAAGTMCTCGTGTTGCAYYCC | 26 | 94…119 | 40.7–42.2 | 5′-Alexa Fluor 488 |
| 23 | 5SrDNA_gym_4 | CTCYYGGGAAGTCCYRRTRTYGCACCC | 27 | 92…118 | 41.9–44.8 | 5′-Alexa Fluor 488 |
| 24 | 5SrDNA_cranial_4 | CYGCCTGGGAATACCRGGTGYYGTARG | 27 | 91…117 | 41.9–48.6 | 5′-Alexa Fluor 488 |
| 25 | Tel_UniOP_Arabidopsis | TTTAGGGTTTAGGGTTTAGGGTTTAGGGT | 29 | na | 40.27 | ATTO425 |
| 26 | Pgms1 | ACATTCTTGATACATTCTTGATACATTCTT | 30 | na | 38.13 | 5′-Cy3 |
*At 2 × SSC and 50% Formamide.