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. 2018 May 23;9:519. doi: 10.3389/fphar.2018.00519

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Copyright © 2018 Zheng, Huang, Huang, Yang, Zhu, Wang, Wang, He, Ji, Wang, Qi, Liu and Lu.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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SHK-induced apoptosis depending on ROS generation. (A) The proportions of apoptosis cells of A549 were determined by flow cytometry using FITC annexin V/PI double staining assay after SHK treatments. (B) Expressions of Bcl-2, Bax and Cleaved Caspase-3 were measured by Western blot after SHK treatments. (C) The proportions of apoptosis cells of A549 were determined by flow cytometry after NAC pretreatment and SHK treatment using FITC annexin V/PI double staining assay. ^∗P < 0.05, ^∗∗P < 0.01, ^∗∗∗P < 0.001 vs. Ctrl (control), ^###P < 0.001 vs. SHK.

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