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. 2018 May 23;9:519. doi: 10.3389/fphar.2018.00519

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Copyright © 2018 Zheng, Huang, Huang, Yang, Zhu, Wang, Wang, He, Ji, Wang, Qi, Liu and Lu.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Inhibitory effects of SHK in A549 xenograft mice model. (A) Average body weight curves of Balb/c-nude mice (n = 7). (B) Tumor volume ((L × W²)/2, L, long diameter; W, wide diameter) were analyzed. (C) Tumors were photographed and tumor weights were analyzed. (D) Images of senescence cells in tumor consecutive frozen sections using SA-β-Gal staining assay (scale bar: 50 μm). (E) Images of tumor p-H2A.X, p53 and p21^waf protein expressions were presented by immunohistochemistry (scale bar: 50 μm). ^∗P < 0.05, ^∗∗∗P < 0.001 vs. Ctrl (control).

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