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. 2018 May 23;9:1132. doi: 10.3389/fimmu.2018.01132

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Copyright © 2018 Figueiredo, Azevedo, Mousdell, Resende-Lara, Ireland, Santos, Girola, Cunha, Schmid, Polonelli, Travassos and Mielgo.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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C36L1 counteracts the pro-tumorigenic activity of macrophages (MOs) induced by melanoma derived factors. Left: Schematics describing the workflow of the tumor cell proliferation assay. Tumor cells are exposed to either conditioned media from: untreated MOs (MCM1), MOs exposed to tumor-conditioned media (TCMs) from metastatic melanoma B16F10 cells (MCM2), or MOs exposed to C36L1 peptide + TCM from B16F10 cells (MCM3). Next, macrophage conditioned media (MCM) generated from these three conditions were added into B16F10 melanoma, cells and the number of live proliferating cells was quantified by flow cytometry after 72 h. Right: Bar graph represents average of three independent experiments (n = 3). Values represent means ± SEM, and data were analyzed using a two-tailed unpaired t-test (***p < 0.001).