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. 2018 May 29;9(6):654. doi: 10.1038/s41419-018-0695-6

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© The Author(s) 2018

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Fig. 6 — a Gene Set Enrichment Analysis (GSEA) was performed to exploring FSTL1 effect in CRC. (i) KEGG-Focal adhesion, normalised enrichment score (NES) = 2.019, nominal P value (NOM P) = 0.000, false discovery rate (FDR) = 0.063. (ii) KEGG-Regulation of actin cytoskeleton, NES = 1.893, NOM P = 0.000, FDR = 0.080. (iii) PID-Integrin1 pathway, NES = 1.631, NOM P = 0.004, FDR = 0.407. (iv) KEGG-Focal adhesion, NES = 1.697, NOM P = 0.026, FDR = 0.464. b Western blotting analysis was performed to detect the expression of FSTL1 and the main members of focal adhesion signalling pathway in indicated stable cells. Phosphorylated FAK at Tyr-397, Phosphorylated Paxillin at Tyr-118, and Phosphorylated SRC at Tyr-418. c Immunofluorescent staining was performed to observe the expression of FAK (green, upper panel), p-FAK (green, lower panel) and actin cytoskeleton in DLD1-Vector and DLD1-FSTL1 cells (×1800, scale = 10 μm). Phalloidin (red) stains F-actin, DAPI (blue) stains nuclei. Right column are the partial enlarged details from the white pane of corresponding original images