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. 2018 May 29;8:8317. doi: 10.1038/s41598-018-26710-z

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© The Author(s) 2018

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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MmpL11 is essential for Mtb to grow in fatty acid rich conditions: (A) Bacterial growth in the presence of different concentrations of methyl palmitoleate with (solid symbols) and without ADC (open symbols) for 15 days is depicted as % relative growth mean ± SD of triplicate wells of one of three experiments. Values are represented as ratio of mean O.D. at 600 nm in methyl ester of fatty acids relative to no treatment ± SD. (B) The bacterial numbers at 0, 1, and 3 days of growth in media containing 33.5 µM methyl-palmitoleate for the Mtb strains is shown as mean cfu/ml ± SD. (C) Growth in the presence of methyl oleate or methyl elaidate with (solid symbols) and without ADC (open symbols) is depicted as the average % relative growth mean ± SD of triplicate wells. (D) Quantitation of CL in the lipids of Mtb grown in methyl-oleate for 24 hr by densitometry. The relative amount of CL is represented as mean ± SD for a representative of 2 biological replicate experiments. (E) Intracellular growth of Wt, ΔM11 and Comp grown in oleic acid pre-treated differentiated THP1 cells is shown as cfu/ml ± SD of triplicate wells of one of the two experiments. (F) A representative image of the bacterial colonies from oleic acid treated THP1 cells at day 1 p.i. for the 3 Mtb strains.