Skip to main content
. 2018 May 23;8:172. doi: 10.3389/fcimb.2018.00172

Figure 3.

Figure 3

OmpAVac induces a multifactorial immune response in mice. (A) The bar represents the titer of anti-loop1, anti-loop2, anti-loop3, anti-loop4, and anti-OmpAVac IgGs from OmpAVac-immunized mice. *indicates a significant difference (P < 0.05), while “n.s.” indicates no significant difference. (B) Comparison of the subtypes of anti-loop1, anti-loop2, anti-loop3, anti-loop4, and anti-OmpAVac IgGs from OmpAVac-immunized mice. The OD at 450 nm in each ELISA reaction is shown. *indicates a significant difference in the OD among IgG1, IgG2a, and IgG2b. (C) Proliferative activity of mouse splenocytes after in vitro stimulation with loop1, loop2, loop3, loop4, and OmpAVac for 72 h, respectively. Proliferation was measured using the bromodeoxyuridine (BrdU) labeling method. *indicates a significant difference, while “ns” indicates no significant difference. “#” indicates a significant difference between the OmpVac-stimulated group and the other groups. (D) Cytokine production by antigen-stimulated splenocytes from OmpAVac-immunized mice. Two weeks after the final immunization, the spleens were processed and stimulated with loop1, loop2, loop3, loop4, and OmpAVac for 72 h, and the levels of IL-4, IFN-gamma, and IL-17 in each culture supernatant were measured. The data are shown as the means ± SE and the significance of the differences was determined by unpaired parametric tests (Student's t-test for two groups or one-way ANOVA for three or more groups). *indicates a significant difference (P < 0.05).