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. 2018 May 23;9:543. doi: 10.3389/fphar.2018.00543

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Copyright © 2018 Wang, De, Yue, Ma, Cheng, Zhu, Du, Yi, Zhao and Chen.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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The effect of various PTX formulations on signaling of metastasis related proteins in 4T1 cells in vitro. (A) Western blotting analysis of the expression of uPA, Bcl-2, twist, Survivin, CD44, MMP-2 and MMP-9 on treated 4T1 cells, with actin as control. (B) Relative intensities of the expression of the proteins above in the cells after treatment by incubation with various PTX formulations for 24 h. (C) qRT-PCR determination of mRNA levels of uPA, Bcl-2, twist, Survivin, CD44, MMP-2 and MMP-9 on 4T1 cells after treatment by PTX, PELA-PTX, and PELA-PBAE-PTX micelles.