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. 2018 May 23;12:139. doi: 10.3389/fncel.2018.00139

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Copyright © 2018 Li, Song, He, Chen, Wu, Li, Bai, Liu, Li, Wang, Han, Xu, Zhang, Li, Chai, Wang and Fan.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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TEM was used to determine cell morphology in (A) the undamaged HEI-OC1 cells, (B) after cisplatin treatment for 24 h, and (C) after cisplatin treatment for 48 h (arrows in the pictures indicate the autophagic lysosomes). (D) Western blot results showing LC3-II expression changes after different exposure times (0, 3, 6, 12, 24, and 48 h). β-actin served as the sample loading control. (E) Quantification of the western blot in d. ^∗p < 0.05 compared with the control. n = 3. Scale bars = 2 μm.