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. 2018 May 29;8:8248. doi: 10.1038/s41598-018-26607-x

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© The Author(s) 2018

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

PMC Copyright notice

Entropy analysis of miRNAs. Shannon’s entropy was used to quantify miRNA diversity for developmental stages through adulthood. We observed an increase of diversity at early developmental stages (2 to 5 dpf), where cell differentiation and organ formation occurs, followed by stabilization in late larval (10 dpf) and adult (6 month years old) stages. Developmental stage labels and images were obtained from Fujimura and Okada, 2007 under Creative Commons Attribution 4.0 International (CC BY 4.0 - authors “Koji Fujimura and Norihiro Okada”, title “Fig. 1. Overview of the developmental stages of Nile tilapia O. niloticus.”, date “14 March 2007”, url “http://onlinelibrary.wiley.com/doi/10.1111/j.1440-169X.2007.00926.X/full”, no endorsement, only esthetical adaptations: image vectorized, background removed, shadow included and image rotated. Adult Oreochromis niloticus image from https://www.usgs.gov/ under the Public domain.