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. 2018 May 29;8:8212. doi: 10.1038/s41598-018-26404-6

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© The Author(s) 2018

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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NPCC isolation simply activates PDX1⁺/SOX9⁺/multi-hormonal pancreatic progenitor-like cells in cultured NPCCs. IFA for (A) PDX1 (green)/insulin (red) and (C) SOX9 (green)/E-cadherin (red) in NPCC cultures. Quantitative results showed a significant increase of (B) PDX1⁺ and (D) SOX9⁺ cell populations in freshly-isolated NPCCs, compared to neonatal pig pancreas, and maintained high percentage over 4 days in culture. NPCCs from 1- to 2-day neonatal pigs showed significantly more progenitors than those from 3-day new-born pigs; (E) Rare but reproducible multi-hormonal progenitor-like cells (arrows) in 2-day NPCC cultures isolated from 1-day neonatal pigs was detected by IFA. DAPI was used to localize cell nuclei. ***p < 0.001.