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Killing of G. mellonella larvae infected with E. faecalis OG1RF and ΔclpB at 37 °C. Survival (Kaplan–Meyer) plots of larvae injected with OG1RF (•) and ΔclpB (▪) strains. Larvae injected with saline solution (▾) or heat-killed OG1RF (⧫) were used as controls with minimum killing. The experiments were repeated three times, and the results are representative of a typical experiment. Compared with the wild-type OG1RF strain, ΔclpB showed attenuated virulence (P≤0.001).
