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. 2011 Mar 1;157(Pt 3):656–665. doi: 10.1099/mic.0.041897-0

Table 2.

Oligonucleotide primers and probes used in this study

The underlined bases correspond to restriction sites included to aid in the subsequent cloning of the PCR products.

Primer name Sequence (5′–3′) Application
FW clpB UP GGAGGATGG AATTCTCGCTTTTCT clpB deletion
RV clpB UP TGAGCTTCACCCGGGGCCTCTTGA clpB deletion
FW clpB DOWN GAAGGTGTGCCCGGGGAAGGAAC clpB deletion
RV clpB DOWN AACCCTTGGGCATGCGACAGACAA clpB deletion
FW ctsR UP CTTTGCAAAAGGATCCGCG ctsR deletion
RV ctsR UP AGCCTCAAGAATTCCTGACGTATT ctsR deletion
FW ctsR DOWN GGAAGGCAAGAATTCGCTAGCTG ctsR deletion
RV ctsR DOWN CCAACTACTGGATCCAAAC ctsR deletion
clpB forw 3 CAAAGGATCCAAGAGTTGGTCAAAC ΔclpB complementation
clpB rev 3 GAATTCCGTCTCGAGTTACACTTC ΔclpB complementation
clpB forw 2 GATGCTGGTTTAGATGTTGACG qRT-PCR
clpB rev 2 CGAAGTGAATCAGCTTCTTGC qRT-PCR
16S RNA-F CGCTAGTAATCGTGGATCAGAATG qRT-PCR
16S RNA-R TGTGACGGGCGGTGTGTA qRT-PCR
clpB forw 1 ATCATTGGTCGTGACGAA clpB probe
clpB rev 1 ATTCACTCATGTCAATCC clpB probe