Table 2.
Oligonucleotide primers and probes used in this study
The underlined bases correspond to restriction sites included to aid in the subsequent cloning of the PCR products.
| Primer name | Sequence (5′–3′) | Application |
|---|---|---|
| FW clpB UP | GGAGGATGG AATTCTCGCTTTTCT | clpB deletion |
| RV clpB UP | TGAGCTTCACCCGGGGCCTCTTGA | clpB deletion |
| FW clpB DOWN | GAAGGTGTGCCCGGGGAAGGAAC | clpB deletion |
| RV clpB DOWN | AACCCTTGGGCATGCGACAGACAA | clpB deletion |
| FW ctsR UP | CTTTGCAAAAGGATCCGCG | ctsR deletion |
| RV ctsR UP | AGCCTCAAGAATTCCTGACGTATT | ctsR deletion |
| FW ctsR DOWN | GGAAGGCAAGAATTCGCTAGCTG | ctsR deletion |
| RV ctsR DOWN | CCAACTACTGGATCCAAAC | ctsR deletion |
| clpB forw 3 | CAAAGGATCCAAGAGTTGGTCAAAC | ΔclpB complementation |
| clpB rev 3 | GAATTCCGTCTCGAGTTACACTTC | ΔclpB complementation |
| clpB forw 2 | GATGCTGGTTTAGATGTTGACG | qRT-PCR |
| clpB rev 2 | CGAAGTGAATCAGCTTCTTGC | qRT-PCR |
| 16S RNA-F | CGCTAGTAATCGTGGATCAGAATG | qRT-PCR |
| 16S RNA-R | TGTGACGGGCGGTGTGTA | qRT-PCR |
| clpB forw 1 | ATCATTGGTCGTGACGAA | clpB probe |
| clpB rev 1 | ATTCACTCATGTCAATCC | clpB probe |